Chorionic villus-derived mesenchymal stem cell-mediated NRG1 upregulation promotes HTR-8/SVneo cells proliferation through the activation of the NF-κB signaling pathway

In a prior study, our group found that chorionic villus-derived mesenchymal stem cells (CV-MSCs) were capable of promoting trophoblast proliferative and invasive activity. The mechanistic basis for this activity, however, has yet to be clarified. As such, an RNA-Seq analysis was conducted using trophoblasts treated with or without CV-MSC-conditioned media. Of the differentially expressed genes identified when comparing these two groups cells, 23 proliferation-associated knocked down test their functional roles in trophoblasts. These analyses revealed inhibiting neuregulin 1 (NRG1) expression sufficient suppress proliferation induce cell cycle arrest Placental samples from patients preeclampsia exhibited significantly increased NRG1 relative healthy pregnancies. Following treatment media, upregulated at mRNA protein levels. Relative control trophoblasts, those which had been impaired DNA replication inactivation NF-κB signaling pathway. contrast, overexpressing yielded opposite phenotypes. Even NRG1, inhibition (P < 0.05). results indicate elevated may play role ability CV-MSCs activity through axis.